Exposure to non-self red blood cells (RBCs) and platelet (PLT) antigens during transfusion or pregnancy can lead to the development of alloantibodies, that can cause clinically significant and even fatal complications. Therefore, antigen typing of both recipient and donor RBCs and platelets is vital and improves transfusion practice and outcomes. Current clinical methods for antigen typing use serology and molecular detection of single nucleotide polymorphisms (serology/SNPs), but these have limitations. To overcome these limitations, we are developing new typing methods using next generation sequencing (NSG).
There is an expanding array of biologic therapies that can be used to treat autoimmune diseases, including B cell depletion, T cell costimulation blockade, and neutralization of specific cytokines (TNF, IL-1, and others). However, there are no tools to help predict which therapy will work best in an individual patient. We are using high-dimensional analyses of patient blood and tissue samples to identify active immune pathways in individual patients that guide selection of effective therapies.
The immune system, and its regulation is the center to a wide range of diseases including autoimmune diseases, cancer, infectious, and even metabolic diseases. Dysregulation of CD4+ T cells leads to greater risk of rheumatoid arthritis, type I diabetes, and tuberculosis. Each of these diseases affect different populations worldwide – but confer disability, morbidity, and mortality even with state of the art treatment.
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